Catalog Number - 85518-15P

The 85518‑15P Cogent RP C18 ABX™ HPLC Column is engineered for maximum‑resolution analytical separations under extreme mobile phase conditions, delivering reliable, reproducible performance across a wide pH range (2–12).

Constructed with a bulky, specially end‑capped C18 phase bonded to high‑purity silica, this column minimizes secondary interactions and improves peak shape for acidic, basic, hydrophobic, and neutral analytes, including compounds with very high or very low pKa values.

✅ Ideal as a USP L1 alternate for demanding acid/base methods

✅ One of the most popular RP C18 ABX™ columns for high‑resolution analytical workflows

150 mm Length – Maximum Resolution for Analytical Methods

• Highest resolving power in the 5 µm ABX analytical column range

• Maximizes peak capacity for complex mixtures

• Ideal for closely related compounds and difficult separations

• Preferred for final method development and validation workflows

✅ Selected when maximum separation performance is required beyond 50 mm and 100 mm formats

4.6 mm ID – Standard Analytical Platform

• Compatible with standard HPLC systems

• Ideal for:

  • QC and regulated laboratories

  • method development and validation

Typical Flow Rate Range

• ~0.8–1.5 mL/min

✅ Provides robust, reproducible analytical performance

5 µm Particle – Maximum Durability & Method Transferability

• Provides:

  • Long column lifetime

  • Excellent robustness under routine use

  • Lower backpressure vs smaller particle sizes

✅ Ideal for:

• Validated methods and routine QC workflows

• Method transfer between laboratories

• Systems with pressure limitations

Wide pH Stability (pH 2–12) – Extreme Method Capability

• Designed for acidic and basic mobile phase conditions

• Suitable for:

  • Strong acids

  • Strong bases

• Maintains integrity under harsh analytical environments

✅ Enables separations beyond standard C18 column limitations

Why Choose RP C18 ABX™ Columns (5 µm)

Extreme pH Ruggedness

• Stable across full pH range (2–12)

• Ideal for stability‑indicating and aggressive analytical methods

Maximum Reliability & Reproducibility

• Designed for consistent results across instruments and labs

• Supports validated and regulated workflows

Versatile Analytical Performance

• Suitable for:

  • Hydrophobic compounds

  • Neutral analytes

  • Long‑chain fatty acids

✅ Preferred for routine, high‑reliability analytical applications

Application Performance Advantages

• Enables:

  • Reliable retention and resolution

  • Improved performance over legacy 5 µm columns

• Comparable to other high‑pH C18 phases used in regulated environments

✅ Ideal for method validation and long‑term analytical consistency

System & Sample Considerations

• Compatible with standard HPLC systems

• Suitable for routine and high‑throughput workflows

Recommended sample preparation:

• 👉 Use 0.2 µm syringe filters for proper sample preparation before injection

✅ Helps:

• Prevent column fouling

• Maintain long‑term performance and reproducibility

Typical Applications

• HPLC analytical separations

• Acidic and basic compound analysis

• Pharmaceutical QC and validation

• Stability‑indicating methods

• Complex mixture separations

• Routine analytical workflows

Important Usage Notes

• Designed for standard analytical HPLC systems (4.6 mm ID)

• Suitable for extreme pH mobile phases (pH 2–12)

• Ideal for maximum‑resolution analytical workflows

• Monitor system pressure based on instrument specifications